Plasmid

Part:BBa_K5322032:Design

Designed by: Xinquan Zhou   Group: iGEM24_NJTech-China   (2024-10-01)


Lpp-OmpA-Programmed Cell Death 1 Ligand 1 Functional Domain [Mus musculus] Expression System


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 832
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 790
    Illegal NheI site found at 1415
    Illegal PstI site found at 832
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 681
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 832
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 832
    Illegal NgoMIV site found at 1221
    Illegal AgeI site found at 1053
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The full-length mouse PD-L1 protein has a large molecular weight and a complex structure. Therefore, we referred to the descriptions of mouse PD-L1 in the NCBI database (ADK70951.1, ADK70950.1, Q9NZQ7.1, AAH66841.1) and truncated the Programmed Cell Death 1 Ligand 1 [Mus musculus] (BBa_K5322033). The functional domain—the immunoglobulin variable (IgV) domain—corresponds to residues 20 to 130 in the 290 amino acid sequence. To ensure that the functionality of the truncated protein is preserved, we retained the first 19 amino acids and the last 20 amino acids (131-150), aiming to minimize any impact on the IgV structure.


Source

[Mus musculus] NCBI：ADK70951.1, ADK70950.1, Q9NZQ7.1, AAH66841.1


References